What is Acetylcholine and how does it produce contraction of the smooth muscle (dynamics: receptors,type and drug-receptor interaction)?

Data analysis and interpretation (up to 1700 words): (40%)

1. Discuss
What is Acetylcholine and how does it produce contraction of the smooth muscle (dynamics:
receptors,type and drug-receptor interaction)?
What is pilocarpine  (or carbachol if it was chosen in the experiment) ? what is its mechanism of action? discuss briefly its therapeutic applications
Identify two drugs that can be used for their effect on smooth muscles within the body (urinary tract, blood vessels, or respiratory system) and briefly discuss the following:

The mechanism of action
Drug-drug interaction
Therapeutic application

Compare the 2 dose-response curves  (without antagonists): which agonist is the most potent? And which one is the most effective? provide evidence from your data.

Provide an approximate value of ED50 for both acetylcholine and pilocarpine or carbachol.

Analyse the effect of the antagonists on the dose-response curves for both drugs. How would you describe the mechanism of action of the antagonist on the muscarinic receptors?