What happens to the longevity of worms when RNAi is used to knockdown HIF-1 in MIT mutant worms?

Hypoxia – TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants

Instructions:

Answer questions 1-4 by reading the following article. All answers must come from SOLELY from the following journal article . The answers should be 1-2 sentence and it should summarize the answers EFFECTIVELY and CONCISELY in 1-2 sentences unless specified otherwise. In question 1, you may use 4-5 sentences. Make sure the answers are accurate and precise and derived appropriately from the paper. No specific format needs to be use, only the answers need to be given. No citations required.

Ask me any questions for clarification if instructions or questions are unclear. Thanks.

Click on the link to access the article.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838777/

1. (2%) Explain the relevance of testing the phenotype of RNAi treatment for 400 genes on wild type or MIT mutant c. elegans (make sure to address why MIT mut worms were used and what research group(s) discovered this particular MIT mut worm phenotype).

2. (1%) What important discovery does this paper make about TAF-4 and HIF-1?

3. (1%) In worms, approximately how many genes does HIF-1 regulate during hypoxia?

4. (1%) What happens to the longevity of worms when RNAi is used to knockdown HIF-1 in MIT mutant worms?

How did the authors use functional validation to confirm their hypothesized link between Hp1bp3 and cognitive function.

Systems genetics identifies Hp1bp3 as a novel modulator of cognitive aging

Instructions:

Answer questions 1-5 by reading the following article. All answers must come from SOLELY from the following journal article . The answers should be 1-2 sentence and it should summarize the answers EFFECTIVELY and CONCISELY in 1-2 sentences.

Make sure the answers are accurate and precise and derived appropriately from the paper. No specific format needs to be use, only the answers need to be given. No citations required.

Ask me any questions for clarification if instructions or questions are unclear.

Click on the link to access the article.

M., Garfinkel, B. P., Wilmott, L. A., Ignatowska-Jankowska, B. M., Citri, A., Orly, J., Lu, L., Overall, R. W., Mulligan, M. K., Kempermann, G., Williams, R. W., O’Connell, K. M., & Kaczorowski, C. C. (2016). Systems genetics identifies Hp1bp3 as a novel modulator of cognitive aging. Neurobiology of aging, 46, 58–67. https://doi.org/10.1016/j.neurobiolaging.2016.06.008

(1%) The authors refer to CFM testing, what does this acronym stand for and describe what it helps the authors test in this study?

(1%) Explain how the authors use their data to make the connection between Hp1bp3 and Alzheimer’s disease?

(1%) Explain how the authors use their data to link Hp1bp3 to memory capacity in humans?

(1%) Why did the authors use the BXD mice in this study?

(1%) How did the authors use functional validation to confirm their hypothesized link between Hp1bp3 and cognitive function.

Explain the real-World Efficacy of Second-Line Toripalimab in Patients With Advanced NoneSmall-Cell Lung Cancer.

Explain the real-World Efficacy of Second-Line Toripalimab in Patients With Advanced NoneSmall-Cell Lung Cancer

To clarify the real-world efficacy and safety of second-line Toripalimab, we assessed 95 consecutive patients with advanced nonesmall-cell lung cancer in a retrospective multicenter trial.

Nonadenocarcinoma and a large number of metastatic sites correlated with poor progression-free sur vival (PFS). PFS and overall survival (OS) were longer in patients with pembrolizumab-related adverse events; however, PFS and OS were shorter in patients with interstitial lung disease

Discuss the potential difficulties and limitations of the proposed procedures and alternative approaches to achieve the aims.

Neuroscience research proposal outline

1. Introduction

Background and Significance

Briefly sketch the background leading to the present proposal, critically evaluate existing knowledge, and specifically identify the gaps that the project is intended to fill. State concisely the importance and relevance of the research described to the proposed study.

Question(s)/Hypotheses

Address the broader objectives/question and the more specific aims/hypotheses of the proposed research. Have specific predictions about what you will find, including an expected interaction between the variables.

2. Methods

Describe the specific details for carrying out the proposed study, including the population of subjects to be used (see below for use of animal subjects), the stimuli and/or minor equipment needed, and the procedures to be used to accomplish the specific aims of the project. Particularly describe any differences in methodology from previous studies on this topic and their advantage over existing methodologies.

Discuss the potential difficulties and limitations of the proposed procedures and alternative approaches to achieve the aims.

What is the most important non-academic skill in the Hidden Curriculum (definition in CH 10 of the textbook the related PowerPoint) that a doctor or other health professional should have/develop?

Doctors’ Diaries

Watch the 2 parts of the documentary:

Part 1: https://www.youtube.com/watch?v=rfo1nIeoVBs&t=8s

Part2: https://drive.google.com/open?id=0B4x66BLMlUvBOE9FaVQtSWFsODQ

Updated link for Part 2:

https://drive.google.com/file/d/0B4x66BLMlUvBOE9FaVQtSWFsODQ/view?usp=sharing&resourcekey=0-TPEGszYiibWRT0FKrI8s-A

In your opinion, what is the most important non-academic skill in the Hidden Curriculum (definition in CH 10 of the textbook the related PowerPoint) that a doctor or other health professional should have/develop? Explain your reasoning in detail.

Make sure to reference a specific scene (s) from Doctors’ Diaries.

Make sure to define “Hidden Curriculum.”

IMPORTANT

Assessment of paper:

Answering all that is required of the option you select (you can answer all of the questions in any order as long as it is organized in a logical and coherent manner)

Clarity of discussion and analysis

Inclusion of specific and detailed examples from any sources used for the paper (if applicable)

Make sure to proofread your paper for typos and grammatical errors to ensure that your ideas are clearly stated

Grading Rubric:

Clarity of writing (proofread, grammar) = 2 points
Definition = 3 points
Documentary Scene & application of Hidden Curriculum = 5 points

Describe at least one type of illness identity illustrated in the documentary.

Marfan Syndrome Documentary

Watch this documentary: "In My Hands: A Story of Marfan Syndrome"

LINK: https://drive.google.com/open?id=1nwg-AjgXFfFVPizzfE3lsv4i8JLtbOau

Describe at least one type of illness identity illustrated in the documentary.

Include the name of the interviewee, if available, when you provide your example.

Make sure to use the types of illness identities in the CH 7 PowerPoint as a reference.
You may include your general reaction to the documentary.

Chapter 7 powerpoint is included in files.